Universal primers that amplify RNA from all three flavivirus subgroups
نویسندگان
چکیده
منابع مشابه
PCR primers that amplify fungal rRNA genes from environmental samples.
Two PCR primer pairs were designed to amplify rRNA genes (rDNA) from all four major phyla of fungi: Ascomycota, Basidiomycota, Chytridomycota, and Zygomycota. PCRs performed with these primers showed that both pairs amplify DNA from organisms representing the major taxonomic groups of fungi but not from nonfungal sources. To test the ability of the primers to amplify fungal rDNA from environmen...
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A pair of new universal 12S mitochondrial rRNA gene primers was designed through multiple alignment analysis of the mitochondrial tRNA(Phe) and the 5' region of 16S mitochondrial rRNA genes of different kinds of fishes. The primers were successfully used to amplify an expected product fragment of about 1.2 kb from various marine fish species, and the amplified DNA fragment was recognized to con...
متن کاملPrimers to amplify SNP markers in Epichloë canadensis (Clavicipitaceae)1
PREMISE OF THE STUDY Primers were designed to produce short amplicons containing single-nucleotide polymorphisms (SNPs) in β-tubulin (tubB) and translation elongation factor 1-α (tefA) in Epichloë canadensis (Clavicipitaceae), an endophytic fungus of Elymus canadensis (Poaceae). METHODS AND RESULTS Primers to amplify regions of tubB and tefA containing suspected SNPs were designed and tested ...
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ژورنال
عنوان ژورنال: Virology Journal
سال: 2008
ISSN: 1743-422X
DOI: 10.1186/1743-422x-5-16